Robert J. Palmer wrote:
 
>I guess you are interested in bacteria!  We are buying either a BioRad or a
>Leica because we need to examine bacteria on opaque surfaces and therefore
>need the flexibility of upright/invert interconversion.  Leica also have ...
 
>Viel Spass biem Einkaufen!
 
Dear Robert, dear Linda
there are also numerous eukaryotes that are smaller than 10 um ...
 
We are working with yeast, Saccharomyces cerevisiae (Baker's yeast; 5-8 um)
and are very satisfied with the performance of our Leica TCS 4D, which we got
two years ago. Our decision was mainly based on resolution and stability, which
was - at that time - superior to the Zeiss and BioRad systems. Make sure that
there are no vibrations in the system through the laser fan, which, I think, is
still a problem of the design of the Zeiss and BioRad microscopes, when going
to a resolution you need to get with cells as small as yeasts (or bacteria)...
The integration of different applications requiring highest resolution and fast
imaging (Ca measurements) is very difficult and I guess you will have to come
to a compromise, giving up on resolution or speed of image acquisition. If you
are interested in more details, feel free to e-mail me
 
Viel Spass beim Einkaufen!
 
Sepp D. Kohlwein
 
 
 
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Sepp D. Kohlwein, PhD
Genetics and Molecular Biology Group
Department of Biochemistry and Food Chemistry
Technical University Graz                   phone:  ++43 (316) 873-6456
Petersgasse 12                              fax:    ++43 (316) 873-6952
A 8010 Graz, Austria           e-mail [log in to unmask]
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