I use Fluo-3 AM to monitor calcium fluxes in endothelial cells exposed to
flow in a flow chamber mounted on a krypton/argon confocal (Leica).  I need
to extend the experiment time out to an hour.  The fluo-3 is retained, but
it will be necessary to calibrate the signal in different experiments.  I am
planning to use a dual dye loading technique and intend to try CMTMR
co-loaded with the Fluo-3. I know that there is an assumption of similar dye
distributions.
 
Has anyone tried this technique for this purpose?
Any comments on CMTMR or possible alternative dyes that could be suitable?
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Dr. Natalie James
CSIRO Division of Biomolecular Engineering
Riverside Corporate Park
PO Box 184
North Ryde NSW 2113, AUSTRALIA
 
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(Ph) 61-2-886-4885 (Fax) 61-2-886-4818