Dear colleagues,
 
 
 
We have been using confocal microscopy to investigate receptor cycling and
co-localisation of receptors in endosomal and/or lysosomal compartments in
T cell populations.
 
        However we are toying with the idea of sub cellular fractionation using
Percoll , and further analysis of same compartments by gel electrophoresis etc.
Specifically we would like to separate endosomes ( early , late ) from
lysosomes .
 
        We would certainly appreciate correspondence  from any others who have
experience in sub cellular fractionation, on its own or as a compliment to
confocal microscopy.
 
Thank you
                [log in to unmask]
                Dept. of Biochemistry,
                University College, Galway, Ireland
                Tel: +353-91-524411 ext 2431
                Fax:+353-91-525700