If possible, could confocalists help me with another problem.
I would like to measure inracellular pH both spectroscopically and
microscopically, but I require a probe that can be bound to a protein molecule
(eg via amino groups).  I know FITC is normally sensitive but it appears to
loose its sensitivity when bound to my protein of interest.
Any suggestions for alternative probes are gratefully accepted
 
Mark Tobin
Daresbury Laboratory