Hello group.
I'm using a Zeiss microscope to examine a thin film of auto-fluorescent
polyurethane cast onto a glass coverslip. The objective is a Nikon 1.30
glycerol immersion. Images obtained in reflected mode seem reasonable but
transmitted images are sharp only in the periphery. In the center of the
field there seems to be a circular haze extending 1/3 of the way out to
the edge. This occurs at every setting of the condenser, and I can see
the haze through the binocular. Any ideas?
Rik G.