Lets see thing clearly,
 
There are two thype of Zeiss water immersion objectives..
 
The first type are for dipping into a water media and are corrected for use
without cover slips, these objectives are used exclusively  on upright
microscopes, generally fixed stage types..
 
The second type of water immersions objectives have a correction collar for
the useor non use of cover slips, with Zeiss without cover slip is shown as
OD. There objectives can be used on upright or inverted microscopes.
 
The objective that is mentioned as an optimum objective for the Confocal
Microscope is the C- Apo 1.25 na objective and it is designed for use on the
inverted microscope specifically for use with samples that are in a water
media, thus matching the refractive index between the specimen and objective
immersion media
 
For further information contact your local Zeiss Representative for a
specification sheet on Zeiss Objectives.
 
Regards
 
Robert Parsons
Carl Zeiss Canada
 
.Ralf:
>
>We have a 40X water immersion lens for our Zeiss CLSM and we have used it
>extensively.  It is very nice for examination of live cultures in petri
>dishes.  We like it so much that we have placed an order for a 63X water
>immersion.  However, one VERY IMPORTANT note:  our system is based on an
>upright microscope. I cannot see how a water immersion lens can even be
>used for an inverted microscope.  These lenses are specifically designed
>for the upright microscopes to compensate for the small working distance
>when one examines specimen in deep dishes, e.g. petri.  Besides the oil
>immersion oils are always preferable if they can be used.   In addition, it
>seems to me that you already have one of Zeiss's best lenses, the 63X/1.4.
>The currently available water immersion 63X cannot match your 63X oil
>immersion in performance.  So, in my humble oppinion the large sum of money
>for a water immersion lens can be spent better on another enhancement to
>your system which is based on an inverted microscope.
>
>
>>Hello all,
>>
>>a few day ago I was told it would be an improvement to use an water immersion
>>objective on our Zeiss CLSM based on an inverted microscope.
>>At the moment we are using (mainly) a 40x/1.3 and a 63x/1.4 (both oil immer-
>>sion) objective.
>>
>>Has anyone experiences with water immersion objectives? Does it make sense
>>to use them with an inverted microscope or only with upright ones?
>>
>>Thank you for help
>>Ralf
>>--
>>Ralf Steinmeyer                   ([log in to unmask])
>>UNI Hannover                                            Herrenhaeuser Str. 2
>>Inst. f. Biophysik                                      30419 Hannover
>
>Sincerely,
>
>Kamiar Moin, Ph.D.
>Assistant Professor of Pharmacology
>Wayne State University School of Medicine
>Detroit, MI 48201
>
>Tel:  (313)577-0514
>        (313)577-1112
>FAX: (313)577-6739
>E-mail:  [log in to unmask]
>
>