CONFOCALMICROSCOPY Archives

January 1996

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Confocal Microscopy List <[log in to unmask]>
Subject:
? re: aldehyde autofluoresence
From:
Doug Cromey <[log in to unmask]>
Date:
Mon, 22 Jan 1996 13:41:19 -0700
Reply-To:
Confocal Microscopy List <[log in to unmask]>
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Confocalists,
I am aware that aldehyde fixed tissues that are used in the confocal
are prone to autofluoresence (I am aware that given proper post-fixation
treatment, the autofluoresence can be reduced or eliminated).  What is
the chemical basis for the autofluorescence?
 
Thanks.
Doug
.....................................................................
: Douglas W. Cromey, M.S.       Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212A)         P.O. Box 245044                     :
: (voice:  520-626-2824)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  [log in to unmask])  :
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