CONFOCALMICROSCOPY Archives

January 1996

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Confocal Microscopy List <[log in to unmask]>
Subject:
? re: aldehyde autofluoresence
From:
Stefan Gunnarsson <[log in to unmask]>
Date:
Tue, 23 Jan 1996 07:46:15 +0100
Reply-To:
Confocal Microscopy List <[log in to unmask]>
Parts/Attachments:
text/plain (28 lines) 
>Confocalists,
>I am aware that aldehyde fixed tissues that are used in the confocal
>are prone to autofluoresence (I am aware that given proper post-fixation
>treatment, the autofluoresence can be reduced or eliminated).  What is
>the chemical basis for the autofluorescence?
>
>Thanks.
>Doug
 
 
In additition to knowing the chemical basis for the autofluorescence, I
would also be very glad to hear about the proper post-fixation treatment
that reduces aldehyde-induced autofluorescence.
 
TIA
 
Stefan
 
 
..............................................................................
 
Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics
 
Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
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