Recently there have been some questions regarding the use and
     conversion of files to the Bio-Rad PIC image format for use with the
     program Confocal Assistant.
 
     To clarify a couple of points:
 
     The Bio-Rad PIC file format utilizes the ability to store a Z series
     or time series as a single file.  The PIC file itself consists of a 76
     byte header followed by a pixel array of NX x NY x Npic bytes where NX
     and NY are X and Y pixel dimensions of the image and Npic is the
     number of images in the file.
 
     The format of the Bio-Rad header is available from the CoMOS reference
     section of the users manual.  Further information about the Bio-Rad
     PIC file format itself, is available to Bio-Rad Confocal users upon
     request, from myself.
 
     Further user information on the Bio-Rad PIC file format and how to
     convert it to other formats can be found at the following web page
     maintained by Lance Ladic, University of BC Canada:
 
     http://www.cs.ubc.ca/spider/ladic/confocal.html
 
     and then select the Source Code & Tips icon.
 
     Andy Sowerby
     Product Manager
     Bio-Rad Microscopy Division
     Hemel Hempstead UK
 
     e-mail [log in to unmask]
     Telephone 44-1442-232552 xt 297
 
 
 
 
______________________________ Reply Separator _________________________________
Subject: Re: converting TIFF to biorad .pic series
Author:  Confocal Microscopy List <[log in to unmask]> at Internet
Date:    23/01/96 09:26
 
 
On Tue, 23 Jan 1996 14:41:36 +0100 Ralf Steinmeyer wrote:
 
 
>
> I have just tried the Program Confocal Assistant. As I understand
it, to
> work with series in Confocal Assistant a single .pic file has to
contain
> the whole series. Our ZEISS Confocal Microscope saves z-series as
TIFF files
> with one single image of the series in each file, so I think they
have to
> be connected to one .pic file.
>
> Does anyone know how to convert TIFF files taken as a z-series with
a
> ZEISS Confocal Microscope to a Biorad .pic file?
 
Dear Ralph
 
We have had a similar problem. The Biorad file (as I remember it
-others please correct if I'm wrong) has a 512 byte header with lots
of info about the picture etc. To translate the file from Zeiss tiff
you will have to read the Zeiss header to get the resolution info etc.
I have a Zeiss but I don't have the information on the Zeiss header.
Perhaps someone else in the group has this info. in which case I would
like it for future reference also. In any case, a special prgram will
probably have to be written (by someone) to create a pic. file...
 
Regards Mark Cannell