>I don't know if this is an appropriate group, but I was wondering if
>anyone knows about propidium iodide's light sensitivity
>characteristics.  What wavelengths is it sensitive to?  What is the
>decay rate? etc...
>
>thanks,
>Joe
 
Hi Joe,
 
We have used PI a lot for looking at fixed tissues.  Use a standard
rhodamine set (529ex/550em, works well on our confocal) and you should find
that it is very bright and will not bleach significantly.  Email me if you
need specific details about conc. etc.
 
Craig.
 
______________________________________________________________________________
Craig J. Daly
Research Associate                     tel:       0141 330 6606
CRI Heart Failure                      fax:       0141 330 4100
IBLS                                   email:     [log in to unmask]
University of Glasgow                  email:     [log in to unmask]
Glasgow G12 8QQ.
 
Web Site                               http://130.209.48.91/pages/vascular.htm
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