Hi,
   We are about to try the fluorolink Cy5 kit. Have you used these? Are
they as simple to use as the instruction imply? Do you have any
suggestions before we start?
   Thanks,
 
Patty Jansma                                    Tel:520-621-6671
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Arizona Research Labs Division of Neurobiology
University of Arizona
 
 
On Thu, 25 Jan 1996, Peter B. Sargent wrote:
 
> Padraig
> We have used Cy-labeled ligands extensively.  They are extremely bright
> but do bleach rather quickly in aqueous media.  Apparently, if you mount
> in something like methyl salicylate, there's less of a problem.  The
> fluorescence signal may be bright enough to get away with using the 568
> nm line of a KrAr laser to excite (about 11% of max absorbtion), in which
> case bleaching is reduced substantially.
> Jackson immunoreasearch sells many Cy5-labeled reagents, and you can
> label anything they don't sell using reactive dye kits available from
> Amersham/BDS.
> Feel free contact me for more information.
>
> Peter Sargent
> UCSF
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