Dear Jose & Confocalists,
>
> We need some sort of fluorescence resolution pattern that we could
> use to test the resolution of different confocal instruments
> and of deconvolution algorithms.  Does any body know of a source
> that makes such a creature?  We need something that is well characterized,
> i.e. some target that we can be reasonably sure of what we are looking
> at.  e-mail to [log in to unmask] or to the confocal group will be
> greatly appreciated.
The problem with well defined objects is that they must be also easy to come by
and cheap. In the past we have used a line pattern composed of fluorescent
dykes of 100nm width and 300nm height (made by a semi conductors lab at Delft
University), very nice, but one of a kind because of the expense.
As I mentioned in my earlier mail to this group, we now use 140nm or 230nm
latex beads for PSF measurement. For testing our restoration software we use
660nm diameter latex beads which we know to be surface stained. Not a perfect
solution because we do not know the exact fluorescence distribution, but they
are easy to come by and cheap. Contact Jan Bauman ([log in to unmask])
for details: he knows all about it.
 
Bye, Hans
 
 
--
--------------------------------------------------------------
dr. Hans T.M. van der Voort ([log in to unmask])
Scientific Volume Imaging BV,
J. Geradtsweg 181,
1222 PS Hilversum, The Netherlands.
 
URL: http://www.svi.nl/
 
tel: (0)35-6859405 or (0)653-345445
fax: (0)35-6837971