You could do it with layers in Photoshop 3.0 or greater.
 
________________________________________________________________________________
 
 
Paul Goodwin
Image Analysis Lab
FHCRC, Seattle, WA
 
On Tue, 27 Feb 1996, Charles Richardson wrote:
 
> I know that this question was discussed two years ago on this list, but I
> wonder if any updated ideas are now available.
>
> We need to use volume rendering software (in our case, VoxelView on an SGI),
> to reconstruct pathologic lesions which have already been microtomed to their
> glass slides.  We want to digitize the individual slides using a Nikon/Kodak
> DCS420 digital camera on a microscope connected to a PowerMacintosh.
>
> Collect the images, then comes the problem.... Rotating and Translating the
> images to re-register (line them up) in the z-axis.  A classic problem.
> We even have done one with fiducial points to help get the technique down,
> before we move on to more difficult samples.
>
> IDEALLY, I would like to hold one slice image on the screen, then bring
> the next one in the stack up on the screen to overlay the first image, then use
> the mouse to rotate and translate, then "lock" it down when aligned...and so on.
> Probably a pipe dream.
>
> I have heard that a program called "IP Lab" by Signal Analytics Corp. had
> some capability to do this.  Has anyone tried that?
>
> Has anyone else solved the problem of re-registration of histologic specimens?
>
> Thanks in advance for your thoughts and comments.
>
> - Charlie
>
> ---------------------------------------|-------------------------------
> CAPT A. Charles Richardson, DC, USN    | "Almost anything you do will
> Naval Dental Research Inst. Detachment |     be insignificant,
> at the Naval Dental School, Bethesda   |  but it is very important that
> Bethesda, MD 20889-5602                |     you do it."
> Internet: [log in to unmask]      |                     - Gandhi
> ---------------------------------------|-------------------------------
>