Hello Confocalists!
 
Over the last few months there has been illuminating discussion of the
benefits of coverslip-free water / saline immersion lenses... Several
companies now produce fabulously expensive immersion lenses that are
supposed to have highly corrected symmetrical point spread functions. I
understand the theoretical value of such a lens, especially for
deconvolving images, but I am wondering how much improvement they provide
in the real world of living tissues and optically mismatched balanced salt
solutions... Has anyone tried and compared such lenses with more
conventional long working distance immersion lenses? Are they worth paying
3x - 5x more for?
 
Thanks!
IAN
Professor Ian Gibbins
Department of Anatomy and Histology
Flinders University of South Australia
Phone:  +61-8-2045271
FAX:    +61-8-2770085
e-mail:  [log in to unmask]