Hi- We have had excellent success with chloro-methyl-fluorescein diacetate
from Molecular Probes.  We can see it for at least 24 hours after
labelling.  Dave Knecht
 
>Hello,
>
>I am looking for a way to label live cells and maintain the label for 48 -
>72 hours. Previous experiments with PKH26 and fluroescein diacetate did not
>work well, because the dyes were lost before the conclusion of the
>experiment. Now we are looking for an alternative that has to fulfil the
>following criteria: 1.) it should be non-toxic for the cell and should not
>alter its metabolism and 2.) it should stay within the cell for at least 48
>hours.
>I know, it is a tall order, but I has anybody an idea?
>Thank you very much for your help.
 
Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
U-125
Storrs, CT 06269
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