CONFOCALMICROSCOPY Archives

May 1996

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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: plasma membrane dye
From:
"Dr. Gordon Warren" <[log in to unmask]>
Date:
Tue, 14 May 1996 07:47:30 -0500
In-Reply-To:
Dr Steven Thomas <[log in to unmask]> "plasma membrane dye" (May 14, 12:25pm)
Reply-To:
Confocal Microscopy List <[log in to unmask]>
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text/plain (27 lines) 
On May 14, 12:25pm, Dr Steven Thomas wrote:
> Subject: plasma membrane dye
> I am presently tagging intracellular antigens of ovine pituitary
> cells using FITC and TRITC labelled antibodies simultaneously.
>
> I would also like to stain the plasma membranes of the cells to
> determine the relationship between the intracellular labelled
> structures, and the cell membrane.
>
> The fluorochromes will be excited using a krypton/argon laser on a
> BioRad MRC 1000.
 
        DiOC18(3) or DiIC18(3) are good ones to try. See J. Cell. Biol 103:171,
1986 and Cell Tissue Res. 282:311, 1995.
 
 
--
Gordon L. Warren, Ph.D.
Muscle Biology Laboratory
158 Read Building
Texas A&M University
College Station, TX 77843-4243
Fax (409) 847-8987
Phone (409) 862-4809 office
      (409) 764-1658 home
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