CONFOCALMICROSCOPY Archives

May 1996

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: preimmune staining on Testis
From:
Paul Goodwin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 21 May 1996 11:57:00 -0700
Content-Type:
text/plain
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text/plain (38 lines) 
Which critter? Rabbit is natorious for pre-immune antibodies.
 
________________________________________________________________________________
 
 
Paul Goodwin
Image Analysis Lab
FHCRC, Seattle, WA
 
On Tue, 21 May 1996, Simon Watkins wrote:
 
> -- [ From: Simon Watkins * EMC.Ver #2.5.02 ] --
>
> Hi Folks
>
> we are labelling some testis sections for immunofluorescence and get a very
> high seemingly specific labelling with pre-immune serum (not good).  This
> has happened with multiple batches of  preimmune serum. We are wondering if
> this is a general problem and whether there are any nifty steps we might try
> to block this.
>
> Thanks
>
> simon
>
> --
> ---------------------------------------------------
> Simon Watkins Ph.D.
> Director Structural Biology Imaging Center
> Scaife 840
> University of Pittsburgh
> Pittsburgh PA 15261
> Tel:412-648-3051
> Fax:412-648-8330
> http://sbic6.cbio.pitt.edu/sbic.htm
> ---------------------------------------------------
>

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