I'm a french student working with a confocal microscope.
I'm having problems with getting images of fluorescence: there is an
alleviation of the contrast and brightness while I get down into the
stack.
Does anyone have a solution to that problem or can someone give me
references to articles dealing with image processing to correct the
grey levels of my images?
 
Many thanks.
 
========================    Francois ANGOT    ========================
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Groupe de Recherches en Informatique, Image et Instrumentation de Caen
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