I am not sure about the Cell-Tak solution you reference, but I would suggest
contacting Dan at Bioptechs ([log in to unmask]).
Bioptechs makes a great profusion chamber that I believe can accomodate
sterile conditions.
We have had excellent luck with his products in our live cell imaging work.
Regards,
Tom Donnelly
>Anyone knows how to coat the coverslips with Cell-Tak solution and what's the
>optimum concentration to use. Also, I have to culture the cells on the
>coverslip for a few days and so how to keep everything in a sterile condition.
>
>Thank you very much!
>Wing-hung Ko
>
>
Tom Donnelly 206-230-4249 tel
DeltaVision Systems 206-232-4184 fax
Applied Precision, Inc. [log in to unmask]
8505 S.E. 68th St. Web Site: http://www.api.com
Mercer Island, WA 98040