CONFOCALMICROSCOPY Archives

May 1996

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Subject:
Measuring Calcium in Red Blood Cells
From:
Bernard Himpens <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 29 May 1996 21:04:52 +0200
Content-Type:
text/plain
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 Dear Confocalists,
 
We have problems measuring Ca2+ in Red Blood Cells. There is not only a bad
loading with fura-2 or Indo-1 but also a very high background fluorescence.
Fluo-3 is working better but it is harder to quantify.
Has anyone a suggestion to measure Ca2+ in intact cells.
 
Thanks
[log in to unmask]
Laboratory of Physiology
Gasthuisberg O/N
Herestraat 49
B3000 Leuven
ph: +32-16-34 57 27
fax: +32-16-34 59 91

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