CONFOCALMICROSCOPY Archives

July 1996

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Quenching after paraformaldehyde fixation?
From:
"John N. Wright" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 12 Jul 1996 12:29:38 +0600
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        Our cardiomyocyte cell cultures, grown on gelatin coated coverslips
and fixed in 3% paraformaldehyde are lifting off the coverslips after a 30
min./4=B0C treatment with Na Borohydride.  Can anyone suggest a more gentle
quenching protocol to solve this problem without significantly reducing
antigenicity.
Thanks in advance,
John N. Wright
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