CONFOCALMICROSCOPY Archives

July 1996

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Quenching after paraformaldehyde fixation
From:
Larry Ackerman <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 12 Jul 1996 11:02:09 -0700
Content-Type:
text/plain
Parts/Attachments:
text/plain (14 lines) 
Re: question from John N. Wright. It's not clear what you are trying to
quench but I assume it may be aldehyde reactivity with the antibodies. I
never have to do that with formaldehyde based fixation but with
glutaraldehyde based fixation I have been using 2mg/ml glycine as one
component in my blocking solution (which also includes BSA, normal serum
and usually a detergent all in PO4 buffer).
 
Larry Ackerman                                  [log in to unmask]
The Laboratories of Lily & Yuh Nung Jan         Voice (415) 476-8751
Howard Hughes Medical Institute
UCSF, Box 0724, Rm U426                         FAX (415) 476-5774
533 Parnassus Ave.
San Francisco, CA 94143

ATOM RSS1 RSS2