Dear confocalists,
 
I need to determine the centres of gravity (not the geometrical centres) of
foci obtained by confocal microscopy. I realize it is possible to obtain the
geometrical centres using NIH Image, by binarizing the images and skeletonizing.
Is there a procedure (in NIH Image or elsewhere) to automatically obtain the
gravity centres directly from my images, taking into account the grey level
information? Could I get the details?
 
Thanks a lot!
 
Ana
 
 
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Ana Pombo
Sir William Dunn School of Pathology
University of Oxford
South Parks Road                    tel: (+44/0)1865 275527
Oxford OX1 3RE                      fax: (+44/0)1865 275501
UK                                  email: [log in to unmask]