Hello everyone,
 
Would anyone know if the same questions exist for using Fura-2 as the
probe for calcium measurement?
 
 
> 1.  Does anyone know whether aldehyde-related fixation is likely at the
> concentrations of fluo-3 used for calcium measurements?
> What intracellular concentrations of a fluorescein-derived compounds could
> yield aldehydes that would give this problem?
> Does anyone have references discussing this problem?
>
> 2.In addition this factor was suggested as a possible reason for loss of
> contractility in experiments involving scanning of smooth muscle cells.  Any
> comments?
 
Thanks.
 
Paula K Yu
 
 
 
 
 
Paula K Yu