You may wish to look at an article from our laboratory where we calibrated
a number of the theoretical and experimental errors which are present
during volume measurement with the confocal.  The article is:
 
Guilak, F. (1994):  Volume and surface area measurement of viable
chondrocytes in situ using geometric modelling of serial confocal sections,
Journal of Microscopy-Oxford, 173(3): 245-256.
 
Sincerely yours,
Farshid Guilak
-------------------------------------------
 
>Dear all,
>
>I'm interested in making volumetric measurements of cells using a BioRad
>MRC500. What I'd like to do first is calibrate the system to take into
>account spherical abberations...
>
>If anyone has any tips, suggestion or brainstorming ideas it would be
>nice to hear from them...
>
>Stephen Loughborough.
>The University of St Andrews,
>Plant Sciences Dept,
>St Andrews,
>FIFE,
>Scotland.
 
_____________________________________________________________________
 
Farshid Guilak, Ph.D.               Orthopaedic Research Laboratories
Assistant Professor                 Departments of Surgery and BME
E-mail: [log in to unmask]       Duke University Medical Center
URL: http://msrbsgi1.mc.duke.edu    Box 3093, MSRB Rm. 375
919-684-2521, 919-681-8490 (fax)    Durham, North Carolina 27710 USA
_____________________________________________________________________