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December 1996

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Confocal Microscopy List <[log in to unmask]>
Subject:
Loading protocols for BO-PRO-1, PO-PRO-1 and DAPI
From:
Stamatis Pagakis <[log in to unmask]>
Date:
Tue, 10 Dec 1996 18:34:31 +0000
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1.0
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To: Confocal user_group <[log in to unmask]>
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Confocal Microscopy List <[log in to unmask]>
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TEXT/PLAIN (20 lines) 
Hello all.

I would like to try loading these indicators on the wall of a pulmonary
vessel which is made up of endothelium and several layers of smooth
muscle.  Any suggestions/known problems about loading and what concentrations
should I start with?

The vessel wall is about 10 microns thick and most probably the
loading solution has access only to one side of it (the outside).  Loading
temperature could be between room temperature and 37 degrees.

Thank you,

******************************************************************************

Stamatis Pagakis Ph.D                                     [log in to unmask]
University Laboratory of Physiology          [log in to unmask]
University of Oxford, Parks Rd.              FAX:  +44 (0)1865 272469
Oxford OX1 3PT,   United Kingdom             Tel:  +44 (0)1865 272551

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