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December 1996

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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Cardiac fiber nuclei
From:
"Evelyn L. Clausnitzer" <[log in to unmask]>
Date:
Mon, 2 Dec 1996 16:02:24 -0700
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Confocal Microscopy List <[log in to unmask]>
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>We are trying to stain cardiac fiber nuclei with two fluorescent dye:
>YOYO-1 and Acridine-Orange. We have tried in non- and paraffin-embedded
>sections. The problem is we are not getting a high contrast level
>between the nuclei and the backgroung (fiber).
>Does anybody have a similar problem?
>Does anybody know a good solution for it?


We have used Yo-Pro 1 with some success.

Evelyn

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