At 01:02 PM 19/12/96 GMT, Tim Gould wrote:
>Dear confocalists,We need a x40 or x60 UV Ojective with a long
>working distance,to enable us to patch clamp neurones.Could anyone
>actually name and recommend a lens,that does the job of focusing.We
>have two lenses,which our man from Noran is unable to get a good
>focus from (maybe because they are phased lenses),but he is unable to
>say why.
>              THANKS,Tim Gould.
>    p.s.WE ARE USING AN UPRIGHT MICROSCOPE.
>

I would suggest a UV transmitting long working distance saline immersible
lens for your work. I have had great success with the Olympus WPlanFL 40X UV
O.7NA 160mm Tube Length water (saline) immersible lens. This has a working
distance of 3.1mm. On an upright microscope it should prove ideal for your
work. The lens itself is dipped directly into the saline.

See: SH Cody & DA Williams, (1987) A novel organ bath design enables the use
of saline-immersible lenses on inverted microscopes. J. Microsc. Vol 187
Part 1 (in press).

Stephen H. Cody,                                        __    /
Biomedical Confocal Microscopy Research Centre        _/  \__/ \
Department of Pharmacology,                          /          \
University of Western Australia,                    /            \
Nedlands WA 6009,                                   \*  ____     /
Australia.                                           \_/    \_ _/
         email:  [log in to unmask]              __
         Phone:  61 09 346 4569   Fax: 61 09 346 3469         \/