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Tue, 28 Jan 1997 11:38:33 +0100 |
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I'm interested in using the ultra-red dyes TOTO3 and TO-PRO3 (from Molecular
Probes) for the nuclear staining of cells spun on slides. My goal is to
simultaneously visualize rsGFP-PML (488/522), another nuclear antigen
stained by a Texas-red-conjugated Ab (568/605), and nuclear morphology
(TOTO/TO-PRO staining, 647/670) with a 3-PMT Bio-Rad 1024 confocal apparatus.
Does anyone have suggestion/experience on TOTO3 and TOPRO3 dyes for nuclear
staining (fixation, dilution, time of staining..)?
Thanks in advance,
Ildo Nicoletti, MD E-mail: [log in to unmask]
I.M.I.S.O. Perugia University Medical School
Policlinico Monteluce, 06122 Perugia,Italy
Phone +039-75-5783974 Fax +039+75+5783444
www2.med.unipg.it/imagelab
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