You will have a problem if you use too much mountant and don't get rid of
the excess mounting medium from around the coverslip before you nail polish
it.  A simple solution is to flip the slide over onto paper towels for 5-10
minutes after mounting.  The paper will absorb the excess mountant and the
slide will be nice and dry for sealing with nail polish.
I have used Mowiol (polyvinyl alcohol) plus anti-photobleach agents which
hardens, but I find Vectashield far superior.


>I have a customer who is using wet fluorescence mounting protectant. They
>been having trouble with this material contaminating the immersion oil and
>have not been successful in sealing the coverslip to the slide (finger
>nail polish). They've asked if there are any hardening mountants which
>might fuction as well as VectaShield. I'm not aware of any but thought I'd
>ask. Thanks in advance for any responses.
>
>Dan Hogan
>Leica, Inc.
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Judy Drazba, Ph.D.  ([log in to unmask])
Confocal Microscopy Facility, NC-3
The Cleveland Clinic Foundation
9500 Euclid Avenue
Cleveland, OH 44195-5001
Office (216)445-3760
FAX    (216)444-7927