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Date: | Mon, 20 Jan 1997 11:52:34 ... |
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Hello, could you tell me whether FM 4-64 will stain membranes of
prefixed material?
Thanks,
Anna Smallcombe
______________________________ Reply Separator _________________________________
Subject: Re: anyone with experience with FM 4-64?
Author: Confocal Microscopy List <[log in to unmask]> at
Internet
Date: 10/01/97 11:17
Amir,
Several of us at the IMR have used FM 4-64 as a general membrane
stain. We have found it to be much easier to use than other dyes such as
DiI since it is easily solubilized in buffers and also in sea water. We
have stained brain slices, Ascaris sperm and sea urchin embryos. The dye
is blazingly bright, not easily photobleached, and has low background since
the dye appears to be less fluorescent when in solution (not in membranes).
It is also great for looking at living material. Ascaris sperm continued
to crawl and sea urchins continued to gastrulate after staining.
Hope this information helps.
Victoria Centonze Frohlich
Deputy Director, IMR
University of Wisconsin, Madison
Pre-MSA meeting SYMPOSIUM / WORKSHOP
"Applications of Multi-photon Excitation Imaging"
August 9 and 10, 1997
Sheraton City Hotel and Cleveland
Convention Center
For information:
(608) 265-3083 or [log in to unmask]
>Hello list members.
>
>Molecular Probes recently recommended to me FM 4-64 as a membrane dye to
>stain tissue sections. Does anyone have any experience with this? We are
>trying to measure cell volumes of pyloric smooth muscle cells from biopsy
>specimens. We have a noran LSCM attached to an Indy workstation running
>InterVision.
>
>Thanks for any response or advice.
>
>Amir A. L. Bastawrous, MD
>Fellow in Pediatric Critical Care and ECMO
>The University of Chicago
>Department of Surgery
>Section of Pediatric Surgery
>
>(773) 702-6175 Office
>(773) 702-2283 Lab
>(773) 702-2281 Fax
>(773) 702-6800 Pager (7208)
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