Dear Carlos

Can you please mail a copy to me as well, please.  We are new in the confocal
field and are having a lot of fun.  But netherless macros will help.
Got the power macro to work on our Zeiss LSM 410.
Thanks

>
> >         Firstly, I am wondering if anyone has come across any more detailed
> > documentation on the macro language...
> I've got a fairly comprehensive manual from Chris Hunter (Carl Zeiss UK).
> It's not complete, but it's been VERY helpful. I can send you a copy if
> you need it, but I've already made lots of notes on it, so it's
> quite messy. Anyway, if you can't get the original I can mail it to you.
>
> For the rest of the functions you can use two ways of guessing them:
>
> 1-See if you can read into the LSM.EXE file (as if it was a text file) and
> you'll find the functions near the end of it, each one accompanied by the
> type of parameters that should be passed. You won't know what those
> parameters mean, though. Trial and error will help.
> I've got a list of these functions which I can mail to you if you need
> them. Anyway, the list won't tell you what the parameters mean...
>
> 2-use "Record macro" then call some menu functions and see what happens...
> (that works fine for things like Z projection, 3D sections, modify
> sequence, etc.)
>
>
> >         and thirdly (and more specifically), I am wondering if anyone can
> > tell me the command(s) which "grabs" the grey-level intensity value of a
> > pixel, when the x,y co-ordinates for the pixel are known (and in what
> > variable the value is stored). This would appear to be a basic function in
>
> I've found a way which I think it's the only one:
> GetImgLine Vect, line, img, orient
> Will produce a vector Vect whose size is the size of the image (or of the
> ROI if one is active) in the orientation chosen by orient. Vect is a type
> 2 vector.
> (img is the handle of the image that you want to measure - the image
> doesn't need to be displayed for you to measure it)
> If orient = 0 (horizontal line) then make line = y and retrieve the
> intensity at (x,y) as:
> intensity = El(Vect, x)   (forget about using intensity = Vect[x] - it
> never works; you can only access vectors safely with SetEl and El() )
>
> Remember that if you're working in a ROI, the coordinates returned by the
> GetImgLine command will be relative to the ROI.
>
> As you said, the language seems to be extremely powerful. It's a pity
> that it lacks a decent documentation.
>
> Hope it helps
>
> Carlos
>
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Stephan H Coeztee
Electron Microscope Unit
Private Bag 3
Wits
2050
South Africa

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