Colin,

        Please refer to the article Butt et al, 1989. Use of fluorochromes
in the study of fungal cytology and differentiation. Experimental
Mycology. 13: 303-320. If you have any more specific questions please
contact me via email.

Best Regards,
Kirk J. Czymmek
[log in to unmask]

On Wed, 19 Feb 1997, Colin Macqueen wrote:

> Dear Folks,
>         A research group here wants to label fungal aerial hyphae in vivo
> to measure the thickness of the growth. The fungi's integrity whilst
> growing on the agar plate cannot be compromised. Does anyone know of
> fluorescent dyes which can be added to the medium and become incorporated
> in the cell walls or other elements (possibly vacuoles?) within the walls
> to provide us with a much better signal to noise ratio.
> Many thanks, Colin
>
>
>
>
> The Hielander
> Colin Macqueen      [log in to unmask]
> Confocal Facility   The University of Queensland  Fax +61-7-3365-4522
>