>
> On Feb 10,  5:43pm, Rui Malho wrote:
>
> I agree, one noisy pixel could stop the accumulation, while the rest of the
> image is significantly dimmer.. In order to be more quantitative, one can
> check the pixel average variance (or standard deviation) over selected
> parts of the two images.
>
The accumulate-to-peak function would be much more useful if it had smarter
end conditions. For example, one could require that a minimum
percentage of pixels  reach a certain threshold. Or one could look at
various moments of the histogram. And since there is so much time in
the slow scan mode, one could run a median filter on the image (or a
user-selected subset of the image) in order to reduce point noise, and
then test.

This is very similar to the problem of doing auto-exposure. You cannot
solve every case, but you can do pretty well in most cases.

Just another suggestion I would like to see implemented in the
Biorad Lasersharp software... BTW, do the other (ie, Zeiss, Leica,
Olympus, etc) confocal manufacturers do any of the above?

--aryeh
Aryeh Weiss                          | email: [log in to unmask]
Department of Electronics            | URL:   http://optics.jct.ac.il/~aryeh
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