Hello,
Why don't you try acridin orange? It works well with cultured tobacco
cells and is excitable with common filter blocks.
Jean-Pierre Verbelen

On Sun, 2 Mar 1997, Estela S. Rossetto wrote:

>         Hi all,
>
>         I would thank you for some help. I study desiccation tolerant
> (resurrection) plants basically by TEM.
>         Now a colleague and I are interested in studying the vacuoles of
> mesophyll cells from hydrated and desiccated leaves of these plants by
> Confocal Microscopy. My colleague is used to the methods for animal samples,
> but not for plant samples.
>         Would you please suggest us references for methods and for
> fluorescent dyes for vacuoles, or maybe share with us your personal
> experience with this kind of material?
>         Thank you very much for the attention,
>         Estela
>               ____________________________________________________
>                             Estela S. Rossetto, Dr.
> Departamento de Biologia Celular
>                         Instituto de Biologia - UNICAMP
>                        Universidade Estadual de Campinas
>                                    C.P. 6109
>                          13081-970 Campinas SP, Brasil
>                       Tel: 0055 19 2397821 Fax: 0055 19 2393124
>                        e-mail: [log in to unmask]
>               _____________________________________________________
>