CONFOCALMICROSCOPY Archives

March 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Gregory Kowalsky <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 31 Mar 1997 14:09:01 -0500
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On Fri, 28 Mar 1997, Brett Schroeder <[log in to unmask]> wrote:

>At 03:39 PM 3/27/97 -0500, you wrote:
>>We are collecting images simultaneously w/ PMT1 and PMT2...While we can
>>merge them on the screen, we are unable to do anything else with the
>>image....cannot convert to tiff or any other format...

>...Once the files are merged and colored in Confocal Assistant, you will then
>be able to save them as .tif files.

I would suggest also checking NIHImage software (Win95 version) as a converter:

<URL:ftp://zippy.nimh.nih.gov/pub/nih-image/>
<URL:http://www.soils.agri.umn.edu/nih-image/>
<URL:http://rsb.info.nih.gov/nih-image/>

List archives:
<URL: ftp://ftp.soils.umn.edu:pub/info/email-lists/nih-image/>
<URL:http://www.soils.agri.umn.edu/infoserv/lists/nih-image/archives/>

Other possibility is to open the .PIC files as Raw image from Adobe Photoshop:

Open As "Raw" from drop-down formats list
Width x Height 768x512(in our particular case)
Header 76 bytes

Note: we're recording either simultaneously PMT1 and PMT2 (image is
splitted), or each image as separate file, not as stack. I didn't find yet
any way to to force PShop to open .PIC stacks (usually convinient in  the
case of recording time series or Z-series). Also - the question of CoMOS's
LUTs still to be mistery for me.

Also, on Fri, 28 Mar 1997, "Julie R. Hens" <[log in to unmask]> wrote:

>...will run only for the BioRad?

As far as it known to me, Confocal Assistant runs on W3.1/WfW3.11 platform.
There is no more requirements.

Also, on Mon, 17 Feb 1997, [log in to unmask] wrote:
>...How To GET MORE ORDERS For Anything...

FYI: Please do not respond to this post! Anybody who has the original
message(with headers/path etc., not the digest version!), please forward it
to me. Scam and spam have to be cleaned.

--
Quantification is the only right way of living!
<I>Gregory B.Kowalsky NDTP, Member of the HTML Writer's Guild</I>

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