CONFOCALMICROSCOPY Archives

July 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
autofluorescence
From:
Barbara Tolloczko <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 3 Jul 1997 15:28:09 EST5EDT
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Hi all,

We want to look at integrins labelled with FITC-conjugated
desintegrins in small rat mesenteric arteries using argon CLSM. The
tissue has to be fixed. 4% PA, even when followed by sodium
borohydride, produces a lot of nonspecific fluorescence. What other
fixatives can we use (membranes have to be well preserved)?

Thanks in advance

Barbara


Dr Barbara Tolloczko
CF Lab, MCI Research Center
Montreal, Que, Canada
H2X 2P4
tel: (514) 849-5201 ext 2365
fax: (514) 843-2095
e-mail: [log in to unmask]

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