We've been using 1% DABCO in buffered 90% glycerin for our UV dyes.
Vectashield is popular here for visible dyes, but has significant
background with UV excitation.


On Mon, 28 Jul 1997 17:09:29 +0100 [log in to unmask] (Stamatis
Pagakis) wrote:

>Hello --
>
>Does the list have any recommendations for a mounting medium with low
>background fluorescence when used with a UV laser? We do have trouble
>with
>the standard ones.
>
>Thank you,
>
>
>***********************************************************************
>***
>
>Dr. Stamatis Pagakis
>[log in to unmask]
>Confocal and Image Analysis Laboratory
>[log in to unmask]
>Division of Membrane Biology                   Tel: +44 (0)181 9593666
>x 2621
>National Institute for Medical Research        Also at: x2622, x2219
>The Ridgeway, Mill Hill, London NW7 1AA        FAX: +44 (0)181 906 4477

Regards,
Glen MacDonald
Virginia Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA  98195-7923
(206) 616-4156
[log in to unmask]
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