CONFOCALMICROSCOPY Archives

November 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Martin Wessendorf <[log in to unmask]>
Reply To:
Martin Wessendorf <[log in to unmask]>
Date:
Tue, 4 Nov 1997 12:25:14 CST
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Responding to the message of Doug Hubatsch

> I want to scan a large area (spinal cord sections) and have the section
> displayed as one image.  Using a lower power objective doesn't work all
> that well but others have scanned multiple areas using a 10x objective
> and then merged the individual images into one large one.  Can anyone
> fill me in on what is required to do this (other than the electronic
> stage).  In particular I'm interested in knowing what software is the
> best to accomplish this task with.

Doug--

You don't need an electronic stage.  Just take a series of overlapping images at
10x, then use the IBM program "Confocal Assistant" to paste them together.
Alternatively, you could probably do the same thing in Photoshop by placing the
images on different layers while arranging them.

Good luck!

Martin Wessendorf
University of Minnesota

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