Dear William, some people use Congo red to stain starch - I think this
     fluoresces green. For the lipid, You could use Nile red, or DiI or
     some other lipid stains which fluoresce red. For protein, I was going
     to suggest eosin, but that also fluoresces green - so what about
     something like methylene blue which I think fluoresces in the far red?

     Since all these, other than DiI are standard histological stains, you
     should be able to buy them pretty cheaply to try them out. I have
     never tried them together, but I thought the suggestions might help.

     Good luck


     Anna Smallcombe PhD     Applications (Bio-Rad)


______________________________ Reply Separator _________________________________
Subject: triple labeling of food
Author:  Confocal Microscopy List <[log in to unmask]>  at
Internet
Date:    02/12/97 14:11


To all,

I am trying to triple label protein, fat, and starch in  dairy products.
I have access to a  BioRad MRC 1024 with a Krypton/Argon Laser. Does anyone
has ideas on the best ( or any) way to do this?



William R. McManus
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305
1-801-797-1920
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