How do the lines hold up?  Do they vary much in power?  Thanks

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*                                                                       *
*       Anthony Moss                    voice  (334)844-9257            *
*       101 Cary Hall                   fax    (334)844-4065            *
*       Zoology and Wildlife Science    email  [log in to unmask]  *
*       Auburn University                                               *
*       Auburn, AL 36849                                                *
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On Tue, 20 Jan 1998, Schibler, Matthew wrote:

> I have been running a 488/514 argon laser and a 543 Helium neon
> combination for five years in one institution on a Zeiss LSM 410 and a
> second set (same combination) for one year at another institution (on a
> Zeiss LSM 310) after it was used for two years by another group.  So far
> I have not had any problems.
>
> I have not used a 633 nm laser.
>
> My understanding about these lasers is that an argon laser can last from
> 2000-4000 hrs with proper care and that the helium-neon lasers (which
> are comparatively low power) can last up to 10,000 hrs.
>
> Hope this helps.
>
> Matthew J. Schibler Ph.D.
> UCLA Brain Research Institute
> 73-384 CHS 951761
> Los Angeles, CA  90095-1761
>
> (310) 825-9783
> FAX (310) 206-5855
> E-mail:  [log in to unmask]
>
> > -----Original Message-----
> > From: Anthony G Moss [SMTP:[log in to unmask]]
> > Sent: Tuesday, January 20, 1998 4:31 PM
> > To:   [log in to unmask]
> > Subject:      Re: how long should a laser last?
> >
> > I remain mystified by the confocal manufacturer's approach to this.
> > Would
> > it not ALWAYS be better to have long laser life (don't answer
> > obviously
> > rhetorical) so isn't there a better way than having the short-lived
> > Kr-Ar
> > laser, even if it is nifty to use?  So much wasted time and effort
> > with the KrAr combo ..... using 2 lasers seems better to me, the
> > long-lived Ar ion for 488/512 and a second long-lived laser (HeNe ?)
> > for
> > the 543, 633 lines.  At least I'm given to believe they are both
> > long-lived.
> >
> > Even though there could be some difficulty with getting the two lasers
> > aligned, and perhaps with colocalization, it should be a reasonable
> > and solvable problem for all confocal manufacturers, I would think.
> > Zeiss did this with one of their scopes; I don't know the success
> > situation with their scope; I noticed their name mentioned in the last
> > discussion.  Any comment from Zeiss confocalists withthis combination?
> >
> > **********************************************************************
> > ***
> > *
> > *
> > *       Anthony Moss                    voice  (334)844-9257
> > *
> > *       101 Cary Hall                   fax    (334)844-4065
> > *
> > *       Zoology and Wildlife Science    email  [log in to unmask]
> > *
> > *       Auburn University
> > *
> > *       Auburn, AL 36849
> > *
> > *
> > *
> > **********************************************************************
> > ***
>