CONFOCALMICROSCOPY Archives

January 1998

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Confocal Microscopy List <[log in to unmask]>
Subject:
Hemoglobin autofluorescence?
From:
Kathryn Spencer <[log in to unmask]>
Date:
Tue, 6 Jan 1998 17:06:43 -0800
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Confocal Microscopy List <[log in to unmask]>
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Hi There!
        Does anybody know about hemoglobin auto-fluorescence? I have red
blood cells showing up as non-specific background in my Fluorescence In
Situ Hybridization slides. They show up in PMT1 and 3, when excited with
either all lines, or 568, or 647 alone. PMT 2 is clean. Any ideas?


Kathy


Kathy Spencer
Scientific Associate
The Scripps Research Institute
La Jolla, CA  92037
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