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April 1998

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Confocal Microscopy List <[log in to unmask]>
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Thu, 30 Apr 1998 17:10:18 +0100
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Bruce,

Just want to mention that BioRad .pic files can be read directly from a
"DOS" disk onto the MAC without any PC coversions. For example, drag and
drop the files onto your MAC hard disk then open them with NIH Image using
the IMPORT dialog from the File menu. Have the Invert and Calibrate Boxes
checked, then use the Custom option to specify the image size, which you
probably know, and the number of images in the stack. (Don't forget to
allocate enough memory). You must also include the header offset, which is
76 bytes and away you go. After loading, you can save as tif for use in
other programs or platforms. Personally, I like using NIH Image for most of
my image analysis; projections, slices, profile plots are all built in. One
of the best pieces of freeware in existence.

You can anonymous FTP it from zippy.nimh.nih.gov in the pub/nih-image
directory.

Mario M. Moronne, Ph.D.
Innovative Microscopies Group
Life Science Division M/S 6-2100
Lawrence Berkeley National Laboratory
1 Cyclotron Rd.
Berkeley, CA.
94720

ph 510-486-4236
FAX 510-486-5664

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