Udo,

You can't do better than the Zeiss 63/1.4. The limit of resolution for a
lens is determined by the numerical aperture (NA - 1.4 in your case).
Since 1.4 NA is the best one can do for an oil immersion lens, you have
the best lens for your purpose. A lens that magnifies to a greater
extent will not yield better resolution. Just use the Zoom on the
confocal to increase your magnification.

Dave Beebe

Udo Friedrich wrote:

> Dear all,
>
> I am starting to use CLSM for studies in microbial ecology in order
> to address questions of diversity (i.e. I am using rRNA-targeted
> fluorescent probes). In our department we have a Zeiss LSM 410.
> Unfortunately, the largest lens we have is a Zeiss Apochromat 63 /
> 1.4 which seems too small for the bacteria in our systems. Does
> anyone have suggestions on particularly useful lenses?
>
> Udo
>
> -----------------------------
> Udo Friedrich
> AG. Microbiology
> Faculty of Biology/Chemistry
> University of Osnabrueck
> D-49076 Osnabrueck
> Germany
> _____________________________



--
David C. Beebe, Ph.D.,  Cataract Research Center
Jules and Doris Stein RPB Professor of Ophthalmology and Visual Sciences

Professor of Cell Biology and Physiology, Washington Univ. Sch. Med.
Campus Box 8096, 660 S. Euclid Ave.
St. Louis, MO 63110 USA
314.362.1621 (office); 314.747.1588 (lab); 314.747.1405(fax)