Heather;

We offer a set of Calibration slides (3) which cover the range from DAPI to
Cy-7.  Our customers use these slides to calibrate their CCD cameras.

The slides are homogenous and quite bright and do not seem to bleach under
a laser.

Contact me or Linda Absher, for further information.

Regards,

Tom


At 02:14 PM 6/16/98 -0700, you wrote:
>As a new-comer to the wonderful world of confocal microscopy, I am
>amazed at its potential but at the same time I often find
>myself way out to lunch :)
>
>If you haven't already guessed, I'am a novice user, of a Biorad MRC
>600 confocal microscope to be exact.  As part of my masters research
>I am using the microscope to observe fluorescently stained yeast
>cells.  The dye I am using stains viable cells a diffuse green
>(cytoplasm) with red intravacuolar structures.  I would like to
>measure (quantitate) the fluorescence intensity of both colours
>independently (I realize there will likely be some overlap - if
>anyone has any suggestions for dealing with this situation your
>advice would be greatly appreciated).  I have already been advised
>that I will require some non-fading fluorescent standards (red &
>green) that I can use to set up the microscope before testing my yeast
>samples.  I have been told that some plastics (some kinds used to make
>cheap toys) can work very well.  I don't know how true this is or
>what plastics would be good.  I would really appreciate any
>suggestions anyone might have on appropriate standards that I could
>use.
>
>Thanks in advance for any advice offered
>
>Heather Heggart
>M.E.Sc. candidate
>University of Western Ontario, Canada
>[log in to unmask]
>
>
Tom Donnelly                       425-313-4549 tel
Biotechnology Group                425-557-1055 fax
Applied Precision, Inc.            [log in to unmask]
1040 12th Ave. N.W.                Web Site: http://www.api.com/dv.html
Issaquah, WA 98027-8929