I'm guessing you are not limiting your request to confocal.  My experience
with on-the-fly processing systems is that the ones by Hamamatsu (formerly
Argus 10 now Argus20 or 50 if you want calcium/fluor imaging) are really
very nice and extraordinarily powerful.  Butch Moomaw (very nice helpful
guy at Hamamatsu at (210)751-3754 can fill you in.
Dage/MTI makes a really good system too; however I had some trouble with
ease-of-use.  It's my understanding that they have taken this under
consideration in recent versions, but that's only 3rd hand info.  I cannot
remember the name of their instrument, but John Bogen of Dage/MTI can tell
you.  He's in Va, but I can't find his # right now.  Dage is in Michigan
City, Ind, on Roeske ("risky") Blvd.  That should help you find them.
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On Mon, 1 Jan 2001, Mancini wrote:

> This has probably been bashed around previously, but I would appreciate comments (to the board or directly to me) regarding people's experience with various "live imaging" systems that are on the market.  We are planning an equipment grant to address the needs of imaging a variety of cell culture models in the live state, principally aiming for bioluminescent proteins (GFP, BFP, etc.).
>
> Our main interest is in intranuclear movement that preliminary studies suggest require tens of sections (or more) per sec.   Of course there will be probably some loss of resolution, but the dynamics are such that force the need for speed.  Also, versatility with filters/lasers?/etc and excellent phase or DIC is required.
>
> Any comments on systems to recommend/avoid would be greatly appreciated.
>
> Sincerely,
>
> Michael A. Mancini, Ph.D.
> Assistant Professor
> Director, Integrated Microscopy Core
> Department of Cell Biology
> Baylor College of Medicine
> Houston, TX  77030
> 713 798 8952
> [log in to unmask]
>