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June 1998

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Confocal Microscopy List <[log in to unmask]>
From:
Tom Donnelly <[log in to unmask]>
Date:
Fri, 19 Jun 1998 08:20:40 -0700
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Confocal Microscopy List <[log in to unmask]>
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Field of view:   F.O.V. # of eyepiece/ MagObj*Optivar (if present)

Depth of Field in microns= 1000/7*NAObj*MagTotal  + wavelenght of light in
microns/2*(NAObj)2

Above formulae from Carl Zeiss, and I don't know if confocal microscopy
changes these in any way.

Tom


At 05:23 PM 6/18/98 -0700, you wrote:
>Hi, everybody:  I am a new member in this group and brought a question
>with me today.  Could anyone help me on how to calculate the field of
>view for my confocal fluorescence detection system?  My system has a =
>0.5
>NA 20x objective and a 100 =B5m pinhole.  Could any one tell me the =
>field
>of view (depth)?  Or how to calculate it?  Lots of thanks.
>
>Shaorong
>
>Shaorong Liu, Ph. D
>Molecular Dynamics
>928 East Arques Ave.
>Sunnyvale, CA 94086
>Tel. 408 737 4835
>Fax. 408 737 4808
>
>
Tom Donnelly            Applied Precision, Inc.
Sales Director          1040 12th Ave. N.W.
Biotechnology Group     Issaquah, WA 98027-8929
(425)313-4549           Web Site: http://www.api.com/dv.html
(425)557-1055 fax
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