We have the Bioptechs heated stage and objective heater, on a Biorad 600
with an upright Nikon Optiphot2.

The objective heater has been used on its own to maintain cells at 37 C
where the cells were in chambers which wouldn't fit on the heated stage.
Since the objective is the largest heat sink in the system, the objective
heater was the most important part.

It works best on an inverted scope, but since we don't have one (yet) we
fitted it to the 63x objective. We liked it and it kept the cells at 37
just fine.
We used an elastic band around the top part of the heater and objective and
this "high tech" method suggested by Dan worked very well and was "not
messy."

>The Bioptechs objective heater I used a few years ago worked very well.  The
>only complaint I had was that you had to use some black gooey stuff to stick
>the heater to the objective, which made the task of switching to other
>objectives a little messy.   My guess is that Dan Fochts has made
>improvements to the design since then.  He's very responsive to customers.
>
>> ----------
>> From:         Mary Teruel[SMTP:[log in to unmask]]
>> Reply To:     Confocal Microscopy List
>> Sent:         Monday, June 22, 1998 11:55 AM
>> To:   [log in to unmask]
>> Subject:      Bioptechs objective heater/INSTEC warm stage
>>
>> Hi - I was wondering what people thought of the Bioptechs objective
>> heater. I would like to use it in combination with an INSTEC WS60
>> Microscope Warm Stage System to keep my cells at 37deg on an inverted
>> microscope. I view my cells by placing the glass coverslips into
>> reusuable metal chambers (about the size of a Petri dish) purchased from
>> Molecular Probes.
>>     If anyone has had experience with the Bioptechs objective heaters
>> and/or the INSTEC warm stage and wouldn't mind sharing it, I would really
>> appreciate it. Thanks in advance!
>>
>> Mary Teruel
>> [log in to unmask]
>>


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX:   604-822-6089
e-mail: [log in to unmask]