We are interested in labeling lymphocytes which will be infused
back into the animal for analysis at a later time point. We would like to
label the lymphocytes with a fluorescent marker which does not cause
significant alteration in lymphocyte function or activation.  The
particular indicator i.e. the spectral excitation and emission properties
are not so important as long as the compound does not activate the cells
and is stable for up 24-48 hours.
        We plan on removing the spleens and other organs and examining the
tissues for recruitment of the infused labelled lymphocytes. We will do
frozen sections and fluorescent microscopy or dissociate the splenocytes
and perform flow cytometry.
        thanks in advance for any suggestions
        Richard Hotchkiss
        Washington University School of Medicine