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September 1998

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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Wood Fungi Staining
From:
"R.Howard Berg" <[log in to unmask]>
Date:
Mon, 14 Sep 1998 14:55:53 -0600
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Confocal Microscopy List <[log in to unmask]>
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Chitin is a common component of fungal wall polysaccharides and it binds
the lectin wheat germ agglutinin.  Try labeling with WGA fluorescently
tagged with a fluorophore that works at non autofluorescent wavelengths.



>I am starting a project with a wood product researcher who wishes to
>fluorescently label fungal hyphae in decayed wood to determine location,
>number etc. with CLSM.
>
>What is a good, common stain for Fungi?
>
>I might add that the sample has shown enormous auto flurescence in both
>Fitc/Tritc applications.
>
>Bill Monroe
>
>
>Bill Monroe
>EM Center
>Mississippi State University
>(601)-325-3019 Lab
>Fax 325-0246


R. Howard Berg, Ph.D.
Associate Professor
Department of Microbiology
    & Molecular Cell Sciences
Campus Box 526041
The University of Memphis
Memphis, TN 38152-6041

phone (901) 678-4449
fax (901) 678-4457
email [log in to unmask]
www.people.memphis.edu/~hberg/

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